|
#
|
组份 |
用量
|
母液浓度
|
|
1
|
Ca(NO3) ·4H2O
|
1 mL/L
|
15 g/100 mL dH2O
|
|
2
|
KNO3
|
1 mL/L
|
10g/100 mL dH2O
|
|
3
|
MgSO4·7H2O
|
1 mL/L
|
4g/100 mL dH2O
|
|
4
|
β-Na2 glycerophosphate·5H2O
|
1 mL/L
|
2 .5g/100 mL dH2O
|
|
5
|
Vitamin B12
|
0.1ug/L
|
|
|
6
|
Biotin
|
0.1ug/L
|
|
|
7
|
Thiamine HCl
|
10ug/L
|
|
|
8
|
PIV*
|
6ml/L
|
|
|
9
|
HEPES
|
0.5g/L
|
|
|
10
|
Na2SiO3·9H2O
|
0.1g/L
|
|
|
9
|
土壤提取液**
|
30ml/L
|
|
PH调到7.0
*PIV
|
组份
|
浓度
|
|
Na2EDTA
|
0.75g/L dH2O
|
|
MnCl2·4H2O
|
0.041g/L dH2O
|
|
ZnCl2·7H2O
|
0.005g/L dH2O
|
|
Na2MoO4·2H2O
|
0.004g/L dH2O
|
|
FeCl3·6 H2O
|
0.097g/L dH2O
|
|
CoCl2.6 H2O
|
0.002g/L dH2O
|
**土壤提取液
土壤提取液配制方法: 取花园土未施过肥200g置于烧杯或三角瓶中，加入蒸馏水1000毫升，瓶口用透气塞封口，在水浴中沸水加热3小时，冷却，沉淀24小时，此过程连续进行 3次，然后过滤，取上清液，于高压灭菌锅中灭菌后于4℃冰箱中保存备用。