Freshwater Algae Culture Collection at the Institute of Hydrobiology
中文
Contact
Contact Us
  • Phone:0086-27-68780871
  • Fax:0086-27-68780871
  • Email:fachb@ihb.ac.cn
  • Address
Freshwater Algae Culture Collection at the Institute of Hydrobiology
No. 7 Donghu South Road
Wuchang District
Wuhan
Hubei Province
China
Bold 3N Medium
Bold 3N Medium 

 #

组份

用量

母液浓度

1

NaNO3

30 mL/L

10 g/400mldH2O

2

CaCl2·2H2O

10 mL/L

1g/400 mL dH2O

3

MgSO4·7H2O

 10 mL/L 

3g/400 mL dH2O

4

K2HPO4

 10 mL/L

3g/400 mL dH2

5

KH2PO4

10 mL/L

7 g/400 mL dH2O

6

NaCl  

10 mL/L

 1 g/400 mL dH2O

7

PIV*

6mg/L

 

8

土壤提取液**

1 mL/L

 

9

Vitamin B12***

 1ml/L

 

*PIV ( Trace mental solution )

组份

浓度

Na2EDTA

0.75g/L dH2O

MnCl2·4H2O

0.041g/L dH2O

ZnCl2·7H2O

0.005g/L dH2O

Na2MoO4·2H2O

0.004g/L dH2O

FeCl3·6 H2O

0.097g/L dH2O

CoCl2.6 H2O 

0.002g/L dH2O

**土壤提取液
土壤提取液配制方法: 取花园土未施过肥200g置于烧杯或三角瓶中,加入蒸馏水1000毫升,瓶口用透气塞封口,在水浴中沸水加热3小时,冷却,沉淀24小时,此过程连续进行 3次,然后过滤,取上清液,于高压灭菌锅中灭菌后于4℃冰箱中保存备用。
***Vitamin B12

在200mL蒸馏水中加入2.4gHPEPS缓冲液。调节PH值到7.8.加入Vitamin B120.027g。于冰箱内保存。

在往培养基中加时,要在灭菌后加,用0.22um的Millpore一次性过滤器在超净台上过滤。

Copyright© Freshwater Algae Culture Collection of the Institute of Hydrobiology.
Since 1 June 2013