Freshwater Algae Culture Collection at the Institute of Hydrobiology
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  • Phone:0086-27-68780871
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Freshwater Algae Culture Collection at the Institute of Hydrobiology
No. 7 Donghu South Road
Wuchang District
Wuhan
Hubei Province
China
CSI
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 #

组份

用量

母液浓度

1

Ca(NO3) ·4H2O

1 mL/L

15 g/100 mL dH2O

2

KNO3

1 mL/L

10g/100 mL dH2O

3

MgSO4·7H2O

 1 mL/L 

4g/100 mL dH2O

4

β-Na2 glycerophosphate·5H2O

 1 mL/L

2 .5g/100 mL dH2

5

Vitamin B12

0.1ug/L

 

6

Biotin 

0.1ug/L

 

7

Thiamine HCl

10ug/L

 

8

PIV*

6ml/L

 

9

HEPES

 0.5g/L

 

10

Na2SiO3·9H2O

0.1g/L

 

9

土壤提取液**

 30ml/L

 

PH调到7.0
*PIV

组份

浓度

Na2EDTA

0.75g/L dH2O

MnCl2·4H2O

0.041g/L dH2O

ZnCl2·7H2O

0.005g/L dH2O

Na2MoO4·2H2O

0.004g/L dH2O

FeCl3·6 H2O

0.097g/L dH2O

CoCl2.6 H2O 

0.002g/L dH2O

**土壤提取液
土壤提取液配制方法: 取花园土未施过肥200g置于烧杯或三角瓶中,加入蒸馏水1000毫升,瓶口用透气塞封口,在水浴中沸水加热3小时,冷却,沉淀24小时,此过程连续进行 3次,然后过滤,取上清液,于高压灭菌锅中灭菌后于4℃冰箱中保存备用。
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Since 1 June 2013