TAP medium

from Gorman, D.S., and R.P. Levine (1965) Proc. Natl. Acad. Sci. USA 54, 1665-1669.

This is probably the most widely-used medium at present for experimental work. 

Make the following stock solutions:

1. TAP salts 

2. phosphate solution 

3. Hutner´s trace elements (follow this link) 

To make the final medium, mix the following:

2.42 g Tris
25 ml solution #1 (salts)
0.375 ml solution #2 (phosphate)
1.0 ml solution #3 (trace elements)
1.0 ml glacial acetic acid
water to 1 liter

For solid medium, add 15 g agar per liter

Autoclave.

For Tris-minimal medium omit the acetic acid and titrate the final solution to pH 7.0 with HCl