Freshwater Algae Culture Collection at the Institute of Hydrobiology
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  • Email:fachb@ihb.ac.cn
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Freshwater Algae Culture Collection at the Institute of Hydrobiology
No. 7 Donghu South Road
Wuchang District
Wuhan
Hubei Province
China
SE
SE (Bristol’s solution)

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组份

用量

母液浓度

1

NaNO3

1 mL/L

25 g/100mldH2O

2

K2HPO4

1 mL/L

7.5g/100 mL dH2O

3

MgSO4·7H2O

 1 mL/L 

7 .5g/100 mL dH2O

4

CaCl2·2H2O

 1 mL/L

2 .5g/100 mL dH2

5

KH2PO4

1 mL/L

17.5 g/100 mL dH2O

6

NaCl  

1 mL/L

 2.5 g/100 mL dH2O

7

FeCl3. 6H2

1ml/L

0.5 g/100 mL dH2

8

EDTA-Fe   *

1 mL/L

 

9

A5 (Trace mental solution)  **

 1ml/L

 

10

土壤提取液** *

 40ml/L

 

*EDTA-Fe
    1N HCl: 取4.1ml浓盐酸用蒸馏水稀释至50ml。
     0.1N EDTA-Na 2 称取0.9306g 溶解至50ml 蒸馏水中。
     称取FeCl3. 6H2O 0.901g 溶于10ml以上步骤已经配制完成的1N HCl 中,然后 与10ml已经配制完成的0.1N EDTA-Na 2 混合, 加入蒸馏水稀释至1000ml。
**A5 ( Trace mental solution )

组份

浓度

H3BO3

2.86g/L dH2O

MnCl2·4H2O

1.86g/L dH2O

ZnSO4·7H2O

0.22g/L dH2O

Na2MoO4.2H2O  

0.39g/L dH2O

CuSO4. 5 H2

0.08g/L dH2O

Co(NO3)2.6 H2O

0.05g/L dH2O

***土壤提取液
土壤提取液配制方法: 取花园土未施过肥200g置于烧杯或三角瓶中,加入蒸馏水1000毫升,瓶口用透气塞封口,在水浴中沸水加热3小时,冷却,沉淀24小时,此过程连续进行 3次,然后过滤,取上清液,于高压灭菌锅中灭菌后于4℃冰箱中保存备用。
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Since 1 June 2013